Background Information: Chloroplasts are located in the cells in the center of a leaf, called the mesophyll, or “middle tissue”. ... Like all enzyme-driven reactions the rate of photosynthesis can be measured by either. Can you think of a way to how you might measure the gross rate of photosynthesis with this technique? The 5. Post-Lab Questions. A plant is shown in a beaker and test tube which bubbles to indicate the rate of photosynthesis. ... What is an indirect measurement of the net rate of photosynthesis occurring in the leaf tissue? Start studying Photosynthesis lab. Purpose: The purpose of this investigation is to measure the rate of photosynthesis in spinach leaves by measuring the length of time it takes for leaf discs to generate enough oxygen to float. Materials: Sodium bicarbonate (Baking soda) As photosynthesis proceeds oxygen is released into the interior of the leaf which changes the buoyancy--causing the disks to rise. Photosynthesis I: An Assay Utilizing Leaf Disks. Fill In The Table Below With The Results From Your First Experiment. Experiment 3: Measuring the Rate of Photosynthesis with Floating Leaf Discs. One problem in measuring a rate of photosynthesis is that there is a competing process occurring at the same time, cellular respiration, a process that uses oxygen. Calculate the change in volume by subtracting the later gas volume from the earlier gas volume. “The hypothesis was…” a. The rate that the disks sink is an indirect measure of the rate of cellular respiration. Include a brief statement describing why you selected the graphing method you did. Measuring the Rate of Photosynthesis; In this section, students learn how to use the floating leaf disk method to measure the rate of net photosynthesis (i.e. Be sure to clearly indicate the x and y axes, the units used, and the graph title(s). Floating C3 Spinach Discs Lab Concerning Photosynthesis that Determined how Light Intensity Affects the Net Rate of Photosynthesis By Senegar & Mitchell and written by Sandra Monreal Abstract Researchers realize that spinach discs float normally so when infiltrating these discs 3. Compare experimental results to control groups! What Was The Name Of The Plant You Used To Study Photosynthesis? Write a detailed conclusion based on your results. Answer the lab question – What factors affect the rate of photosynthesis in plants? b. In Volume For 5 Minutes Is 0.4 ML For Example, If The Volume At 5 Minutes Is 4 ML. 4. Students can measure the rate over time. Since cellular respiration is taking place at the same time, consuming oxygen, the rate that the disks rise is an indirect measurement of the net rate of photosynthesis. Question: Photosynthesis SHORT ANSWER Experiment 1: Measuring The Rate Of Photosynthesis Lab Results 1. the rate of photosynthesis minus the rate of cellular respiration). What was the name of the plant you used to study photosynthesis? In this virtual photosynthesis lab, students can manipulate the light intensity, light color, and distance from the light source. They compare the rate of net photosynthesis in water vs. a … Calculate the change in volume for each measurement time. “The data show …” a. Steucek, Guy L. Robert J. Hill and Class/Summer 1982. Fill in the table below with the results from your first experiment. Present your results in a graph. Experiment 1: Measuring the Rate of Photosynthesis For the first experiment, what was the change in volume from 00:01:30 to 00:02:00? El Odea Branch 2. Learn vocabulary, terms, and more with flashcards, games, and other study tools. 1985. Print and Web Resources: ! 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